PCR Detection of Microbial Pathogens
نویسندگان
چکیده
منابع مشابه
PCR Detection of Microbial Pathogens
This book covers general challenges of introducing primarily noncommercial PCRs and specific procedures into the laboratory, including sample treatment, extraction protocols, quality and quality assurance, and internal and external laboratory processes. The chapters on specific pathogens illustrate principles that could be applied in many diagnostic laboratories. The editor’s preface to this bo...
متن کاملSpecific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis
BACKGROUND The sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis. METHODS Corneal scrapings from patients were used for Gram stain, culture and PCR analysis. PCR was performed with primer pairs targeted to the 18S rRNA gene. The res...
متن کاملDetection of PCR amplicons from bacterial pathogens using microsphere agglutination.
For rapid and inexpensive detection of polymerase chain reaction (PCR) amplicons, a novel microsphere agglutination assay has been developed. PCR is carried out using biotinylated forward and reverse primers, and the amplified DNA fragments are able to agglutinate streptavidin-coated microspheres (5.7 microm in diameter). Purification of PCR amplicons is unnecessary when initial primer concentr...
متن کاملSiderophore-based detection of Fe(III) and microbial pathogens.
Siderophores are low-molecular-weight iron chelators that are produced and exported by bacteria, fungi and plants during periods of nutrient deprivation. The structures, biosynthetic logic, and coordination chemistry of these molecules have fascinated chemists for decades. Studies of such fundamental phenomena guide the use of siderophores and siderophore conjugates in a variety of medicinal ap...
متن کاملEpidemiology, Detection, and Control of Foodborne Microbial Pathogens
Copyright © 2015 Miguel Prieto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Foodborne microbial diseases are a significant public health threat. They occur in both developed and developing countries with different food i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Emerging Infectious Diseases
سال: 2013
ISSN: 1080-6040,1080-6059
DOI: 10.3201/eid1908.130528